Table 2.
Primer. | (5′→3′) Primer of Sequences |
Size of Production (bp) | Position of Production | Annealing Temperature (°C) |
---|---|---|---|---|
P1 | F: GGAGTGTGGTGGAGGGTAAAA | 778 | −2060~−1282 | 57 |
R: CCTATTCCCAAACCTACTGCCA | ||||
P2 | F: AGTTGAATCACTTTGCTTTACAGT | 845 | −1413~−568 | 55 |
R: ACATCTACTCTGCCTGAGGAAC | ||||
P3 | F: TCACCACCTTTCTGCAGTCTC | 775 | −748~27 | 57.5 |
R: TGAACTTGTGCTCGCTGTGA | ||||
P4 | F: GGGGCGGTTTTTCTTTGACC | 457 | −143~314 | 58 |
R: TGTGTGGTTTAGCCCCAGTC | ||||
P5 | F: GCCATGGTCCTAATGTCACT | 505 | 1063–1568 | 56 |
R: TGGCTCTTCCAGGTTTGACA | ||||
P6 | F: AGGAATTCACTTTCTTCCTGGCTT | 759 | 2747–3506 | 57 |
R: TGCTGTCTCTCTTGTAATGCCT |