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. 2022 Sep 7;9(9):448. doi: 10.3390/bioengineering9090448

Table 2.

The primer sequences, production size, and annealing temperature of PCR amplification for the IL17A gene.

Primer. (5′→3′)
Primer of Sequences
Size of Production (bp) Position of Production Annealing Temperature (°C)
P1 F: GGAGTGTGGTGGAGGGTAAAA 778 −2060~−1282 57
R: CCTATTCCCAAACCTACTGCCA
P2 F: AGTTGAATCACTTTGCTTTACAGT 845 −1413~−568 55
R: ACATCTACTCTGCCTGAGGAAC
P3 F: TCACCACCTTTCTGCAGTCTC 775 −748~27 57.5
R: TGAACTTGTGCTCGCTGTGA
P4 F: GGGGCGGTTTTTCTTTGACC 457 −143~314 58
R: TGTGTGGTTTAGCCCCAGTC
P5 F: GCCATGGTCCTAATGTCACT 505 1063–1568 56
R: TGGCTCTTCCAGGTTTGACA
P6 F: AGGAATTCACTTTCTTCCTGGCTT 759 2747–3506 57
R: TGCTGTCTCTCTTGTAATGCCT