Figure 5.

Dose-response curves obtained in (a) 2D or (b) 3D cell-based assays. HEK293T cells were incubated for 5 h at 37 °C with the TNFα, using BgLuc (solid line) and commercial Luc2P (dotted line) as reporters under the control of NFkB -response element. BL measurements were obtained after the addition of BrightGlo substrate as described in the Materials and Methods section. The experiment was performed in triplicate and repeated at least three times.