TABLE 1.

List of bacterial strains and plasmids used

Strain or plasmid	Relevant genotype, phenotype, or description	Source or reference
Strains
N. gonorrhoeae F62	Full LOS α chain, no β chain	P. Frederick Sparlinga
N. gonorrhoeae WR302		Herman Schneiderb
N. gonorrhoeae PID2		Herman Schneider
N. sicca 4318		Herman Schneider
N. sicca 342		Herman Schneider
N. subflava 4324		Herman Schneider
N. subflava 4325		Herman Schneider
N. subflava 4327		Herman Schneider
N. flavescens 4322		Herman Schneider
N. flavescens 4323		Herman Schneider
N. flavescens ATCC 13120		H. Feldmanc
N. subflava 44		H. Feldman
N. subflava 52		H. Feldman
N. cinerea 32824		Virgina Clarkd
N. cinerea 32165		Virgina Clark
N. lactamica 5841		J. McLeod Griffisse
N. meningitidis 89I		J. McLeod Griffiss
N. gonorrhoeae FA19		William Shaferf
N. gonorrhoeae FA5100		William Shafer
E. coli DH5αMCR	Cloning host strain, F−mcrAΔ(mrr-hsdRMS-mcrBC)	BRLg
N. gonorrhoeae F62 ΔlgtA	239-bp ApoI deletion in lgtA; truncated lactosyl α chain	41
N. gonorrhoeae F62 ΔlgtA ΔlgtE	622-bp BspEI and AgeI deletion in lgtE of F62 ΔlgtA	41
N. gonorrhoeae F62TF#1	pNS44lgtABE transformed into F62 ΔlgtA ΔlgtE; MAb 2-1-L8-reactive mutant	This work
Plasmids
pK18		37
pRFAK2-1	F62 lgtF and rfaK fragment cloned into pK18-up (with uptake sequence)	This work
pNS44lgtABE	44 lgtA, lgtB, and lgtE inserted into pK18	This work
pNS44lgtABΔE	44 lgtA, lgtB, and part of lgtE inserted into pK18	This work
pF62TF#1lgtABE	N. gonorrhoeae F62TF#1 lgt fragment cloned into pK18	This work
pNS44lgtG	44 lgtG inserted into pK18	This work

^aUniversity of North Carolina at Chapel Hill, Chapel Hill. 

^bWalter Reed Army Institute for Research, Washington, D.C. 

^cAlbany Medical Center, Albany, N.Y. 

^dUniversity of Rochester, Rochester, N.Y. 

^eUniversity of California at San Francisco, San Francisco. 

^fEmory Univery Medical School, Atlanta, Ga. 

^gBRL, Life Technologies, Rockville, Md.