Skip to main content
. Author manuscript; available in PMC: 2023 Jul 25.
Published in final edited form as: Curr Biol. 2022 Jun 30;32(14):3059–3069.e7. doi: 10.1016/j.cub.2022.06.011

Figure 1. 12B01 growth on alginate involves formation of multicellular clusters with phenotypically distinct sub-populations.

Figure 1.

A-C) Stages of 12B01 growth on alginate. Scale bars are 5 μm. A) In stage i, single cells slowly form undifferentiated groups. Panels show three examples of stage i groups. B) In stage ii, groups grow and differentiate into layered structures, with a solid shell and a mobile core. Panels show mixing in the center of groups. AV; average intensity projection of time-lapse, showing populations of cells that are motile and not motile (Video S1); STD, standard deviation projection, showing differences in pixel intensity between frames. C) Rupture of clusters leads to hollowing in stage iii. Left: optical section through a cluster, showing absence of cells in center; right: 3D projection, showing structure of hollowed cluster. Additional imaging of 12B01 clusters is presented in Figure S1.