FIG. 2.

IcsA in the soluble periplasmic fraction; Pulse-chase and immunoprecipitation analyses of MBG341. Lane 1, total cellular protein (TCP) after pulse for 10 s. Lanes 2 to 9, periplasmic proteins: lane 2, after pulse for 10 s; lanes 3 to 9, after pulse for 10 s and chase for 5, 10, 20, 30, 60, 120, and 300 s, respectively. (A) Immunoprecipitated with monoclonal antibodies to IcsA; (B) immunoprecipitated with polyclonal antibodies to β-lactamase (β-Lac); and (C) immunoprecipitated with polyclonal antibodies to KdpE. Apparent molecular masses of standard proteins run in parallel are indicated (in kilodaltons). Results from one representative experiment of three are shown.