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Folded state of IcsA in the periplasm and the external milieu. (A) Proteinase K treatment of periplasmic IcsA in a dsbB+ (lane 1) and dsbB mutant (lane 2) background. (B) Proteinase K treatment of IcsA isolated from the culture supernatant of wild-type (wt) Shigella. Lanes: untreated (lane 1), treated with DTT (lane 2) or treated with DTT and then denatured with SDS (lane 3). Sizes are shown in kilodaltons.
