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. 2022 Sep 2;10(9):2168. doi: 10.3390/biomedicines10092168

Figure 2.

Figure 2

Conformational cyclic peptide epitopes have seeding activity and toxicity. (a) Aggregation was tracked hourly (shaking for 30 s prior to each reading) with a ThT fluorescent assay after the addition of α-syn monomers alone (100 mM) or in the presence of BSA-conjugated cyclized or linear CGTKEQGGGG peptide (100 nM). Data are representative of 3 independent experiments. (b) Viability of primary rat dopaminergic neurons treated with α-syn oligomers (0.5 μM) or increasing concentrations of BSA-conjugated cyclized or linear peptide. CTL = neurons incubated with vehicle alone. Mean ± SEM of 6 replicates. Global analysis of data performed using one-way ANOVA followed by Dunnett’s multiple comparisons test. * p ≤ 0.05, *** p ≤ 0.001 vs. CTL (vehicle). No statistically significant difference between linear peptide and CTL or between α-syn oligomers and 1, 5 μM cyclic peptide.