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Immunoblot demonstrating Xcp-dependent heterologous secretion of elastase by P. alcaligenes. Plasmid pPB29 containing the lasB gene encoding elastase of P. aeruginosa was introduced into P. alcaligenes Ps93 (Xcp+) and into its xcpR mutant derivative Ps93R. Cells (C) and supernatant (S) were separated and analyzed by immunoblotting with an antiserum directed against elastase. Cellular and extracellular proteins corresponding to equal culture volumes were loaded.
