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. 2001 Feb;183(3):959–967. doi: 10.1128/JB.183.3.959-967.2001

FIG. 2.

FIG. 2

Secretion of P. alcaligenes lipase by P. aeruginosa. (A and B) Plate assays demonstrating lipase secretion. (A) Plasmid pJRDlipAB, carrying the genes encoding P. alcaligenes lipase and its lipase-specific foldase, was introduced into P. aeruginosa strains PAO1, xcpP to -Z deletion mutant DZQ40, and lipAH deletion mutant PABS1. (B) Plasmid pJRDlipAB was cointroduced with pLAFR3, pAX24 carrying the P. aeruginosa xcpP to -Z cluster, or pLAF600 carrying the P. alcaligenes xcpP to -Z cluster into strain DZQ40. (C) Coomassie brilliant blue-stained gel (lanes 1 to 4) and immunoblot with a monoclonal antibody directed against P. alcaligenes lipase (lanes 5 to 8), using supernatants from P. aeruginosa strains grown in TSB medium. Lanes 1 and 5, PAO1; lanes 2 and 6, PAO1/pJRDlipAB, lanes 3 and 7, PABS1/pJRDlipAB; lanes 4 and 8, D40ZQ/pJRDlipAB. The positions of the P. aeruginosa elastase (LasB) and the P. alcaligenes lipase (LipA) are indicated.