FIG. 6.

Complementation of P. aeruginosa xcpPQ and xcpR to -Z deletion mutants by the P. alcaligenes xcpPQ and xcpR to -Z operons. Plasmids pLAF600SB and pLAF600BH carry the P. alcaligenes xcpPQ and xcpR to -Z operon, respectively. Plasmid pAF2 carries the P. aeruginosa xcpPQ operon. (A) Plate assay demonstrating protease activity. (B) Extracellular elastase activity. Samples were incubated with elastin-Congo red for 3 h. The activities are expressed as the amounts of liberated Congo red (OD₄₉₅) per OD₆₀₀ unit.