FIG. 7.

Complementation of P. aeruginosa xcp mutations by P. alcaligenes xcp genes. Cellular (C) and supernatant (S) proteins corresponding to equal amounts of culture were analyzed by immunoblotting using an antiserum directed against elastase. The xcpR to -Z mutant PAO1ΔRZ contained either pLAFR3 or pLAF600BH carrying P. alcaligenes xcpR to -Z. The xcpP mutant PAO1ΔP contained either pMMB67HE (pMMB), pSB10 carrying P. aeruginosa xcpP, or pMPA2 carrying P. alcaligenes xcpP. Plasmids pMXA8 and pMZA3, carrying P. alcaligenes xcpX and xcpZ, respectively, were introduced into corresponding mutants PAO1ΔX and KS902-503 (xcpZ5).