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. 2022 Aug 24;11(17):e022574. doi: 10.1161/JAHA.121.022574

Figure 3. MFG‐E8 is required for the angiotensin II–induced aortic remodeling.

Figure 3

A, Photomicrographs of hematoxylin and eosin staining of aortic walls. Morphometric analysis of the aortic IMT (P<0.001 for main treatment effect, P<0.001 for main genotype effect, P<0.001 for treatment×genotype, by 2‐way ANOVA) (far right panel) B, Photomicrographs of EVG staining of aortic walls. Quantification of elastin breaks (P<0.05 for main treatment effect, P<0.001 for main genotype effect, P<0.01 for treatment×genotype, by 2‐way ANOVA) (far right panel). C, Photomicrograph of Masson's trichrome staining of aortic walls. Morphometric analysis of intimal medial ECM (blue color) (P>0.05 for main treatment effect, P<0.01 for main genotype effect, P<0.01 for treatment×genotype, by 2‐way ANOVA) (far right panel). D, Photomicrographs of PCNA immunostaining of aortic walls. Quantification of relative PCNA stained nuclei area (P>0.05 for main treatment effect, P<0.01 for main genotype effect, P<0.01 for treatment×genotype, by 2‐way ANOVA) (far right panel). Graph showing mean±SEM with individual animal data points for knockout and WT mice with angiotensin II or saline infusion. *P<0.05, **P<0.01 and ***P<0.001 by Bonferroni post‐hoc tests following 2‐way ANOVA. Scale bar=100 μm. Ang II indicates angiotensin II; ECM, extracellular matrix; EVG, Elastin Verhoeff's–Van Gieson; HE, hematoxylin and eosin; IMT, intimal medial thickness; KO, knockout; L, lumen; M, media; PCNA, proliferating cell nuclear antigen; and WT, wild‐type.