Skip to main content
. 2022 Aug 24;11(17):e022574. doi: 10.1161/JAHA.121.022574

Figure 6. Angiotensin II activation of NF‐κB signaling and its downstream proinflammatory effects require MFG‐E8.

Figure 6

A, Representative western blots of aortic p‐NF‐κB p65, NF‐κB p65, MCP‐1, TNF‐α, and GAPDH. B, Quantitative data of p‐NF‐κB p65 protein abundance (P<0.001 for main treatment effect, P<0.01 for main genotype effect, P<0.001 for treatment×genotype, by 2‐way ANOVA). Graph showing mean±SEM and individual data points for knockout and WT mice with angiotensin II or saline infusion. **P<0.01 and ***P<0.001 by Bonferroni post hoc tests following 2‐way ANOVA. C, Quantitative data of MCP1 protein abundance (P<0.01 for main treatment effect, P<0.05 for main genotype effect, P<0.001 for treatment×genotype, by two‐way ANOVA). Graph showing mean±SEM with individual data points for knockout and WT mice with angiotensin II or saline infusion. ***P<0.001 by Bonferroni post‐hoc tests following two‐way ANOVA. D, Quantitative data of TNF‐α protein abundance (P<0.001 for main treatment effect, P>0.05 for main genotype effect, P<0.05 for treatment×genotype, by two‐way ANOVA). Bar graph showing mean±SEM with individual data points for knockout and WT mice with angiotensin II or saline infusion. ***P<0.001 by Bonferroni post hoc tests following 2‐way ANOVA. E, Photomicrographs of immunostaining of TNF‐α. F, Quantitative data of aortic TNF‐α immunostaining area (%) (P<0.01 for main treatment effect, P<0.05 for main genotype effect, P<0.01 for treatment×genotype, by two‐way ANOVA). Graph showing mean±SEM with individual data for KO and WT mice with angiotensin II or saline infusion. *P<0.05, **P<0.01, and ***P<0.001 by Bonferroni post hoc tests following two‐way ANOVA. G, Photomicrographs of immunostaining ICAM1. H, Morphometric analysis of relative ICAM1 immunostaining area (%) (P<0.001 for main treatment effect, P<0.001 for main genotype effect, P<0.001 for treatment×genotype, by 2‐way ANOVA). Graph showing mean±SEM with individual data for knockout and WT mice with angiotensin II or saline infusion. ***P<0.001 by Bonferroni post hoc tests following 2‐way ANOVA. I, Photomicrographs of immunostaining VCAM1. J. Morphometric analysis of relative VCAM1 immunostaining area (%) (P<0.001 for main treatment effect, P<0.001 for main genotype effect, P<0.001 for treatment×genotype, by two‐way ANOVA). Graph showing mean±SEM with individual data for knockout and WT mice with angiotensin II or saline infusion. ***P<0.001 by Bonferroni post‐hoc tests following 2‐way ANOVA. Scale bar=100 μm. Ang II indicates angiotensin II; ICAM1, intercellular adhesion molecule 1; KO, knockout; L, lumen; M, media; MCP1, monocyte chemoattractant protein 1; MFG‐E8, milk fat globule–epidermal growth factor VIII; MW, molecular weight; NF‐κB p65, nuclear factor‐kappa beta p65; p‐NF‐κB p65, phosphorylated nuclear factor‐kappa beta p65; VCAM1, vascular cell adhesion molecule 1; TNF‐α, tumor necrosis factor alpha; and WT, wild‐type.