Table 1.
Primary Antibodies
| Antibody | Company | Catalog no. | Titer for WB | Titer for IHC |
|---|---|---|---|---|
| Angiotensin II | Sigma | A9525 | 1:350 | |
| AT1 | Santa Cruz | sc‐515 884 | 1:500 | 1:100 |
| AT2 | Abcam | Ab92445 | 1;1000 | 1:100 |
| Collagen I | Santa Cruz | sc‐59 772 | 1:50 | |
| GAPDH | Cell Signaling technology | 5014 | 1:1000 | |
| ICAM1 | Santa Cruz Biotechnology | Sc‐8439 | 1:50 | |
| MCP1 | R&D Systems | AF479 | 1:200 | |
| MFG‐E8 | R&D Systems | AF2805 | 1:1000 | |
| MMP2 | R&D Systems | AF1488 | 1:500 | |
| MT1‐MMP | Thermo Fisher Scientific | PA5‐16514 | 1:500 | |
| NF‐κB p65 | Santa Cruz Biotechnology | sc‐8008 | 1:500 | |
| p‐NF‐κB p65 | Santa Cruz | Sc‐33 020 | 1:500 | 1:50 |
| PCNA | Santa Cruz | sc‐9857 | 1:50 | |
| p‐Smad2 | Santa Cruz Biotechnology | 3108 | 1:500 | |
| Smad2 | Santa Cruz Biotechnology | 5339 | 1:1000 | |
| TGF‐β1 | Santa Cruz Biotechnology | sc‐146 | 1:200 | |
| TNF‐α | Abcam | ab9739 | 1:1000 | 1:100 |
| VCAM1 | Santa Cruz Biotechnology | Sc‐13 160 | 1:50 |
AT1 indicates angiotensin II receptor type 1; AT2, angiotensin II receptor type 2; ICAM1, intercellular adhesion molecule 1; IHC immunohistochemistry; MCP1, monocyte chemoattractant protein 1; MMP2, matrix metalloproteinase type 2; MT1MMP, member type I of MMP activator; NF‐κB p65, nuclear factor‐kappa beta p65; p‐NF‐κB p65, phosphorylated nuclear factor‐kappa beta p65; PCNA, proliferating cell nuclear antigen; SMAD2, mother against decapentaplegic homolog 2; p‐SMAD2 phosphorylated mother against decapentaplegic homolog 2; TGF‐β1, transforming growth factor beta 1; TNF‐α tumor necrosis factor alpha; VCAM1, vascular cell adhesion molecule 1; and WB, western blotting.