Figure 2.
Schematic representation of BiFC models and their complementation pairs. (A) Schematic outline of the KMO and HTT BiFC constructs. flKMO/tKMO were fused C-terminally to N-terminus of CFP (CC) via (GGGGS)2 linker. The HTT BiFC constructs were composed of the N17, polyglutamine (polyQ) and polyproline (polyP) domains fused to either C-terminus or N-terminus halves of Venus (VC or VN). For each of the -VN or -VC HTT BiFC constructs in the illustration there are versions with different polyQ lengths: 25Q-VC, 19Q-VN, 46Q-VN, 97Q-VC and 97Q-VN. (B,C) Illustrations of BiFC combination pairs: (B) KMO and HTT pair that when interacting bring CC and VN together to form a fluorescence protein with 510 nm emission as in enhanced-GFP (E-GFP); (C) HTT pairs upon interactions, the two halves of Venus (VN and VC) re-constitute with emission 527 nm.