Table 1.
Primers used on the hypothalamus qPCR.
| Gene | Primer Sequences (5′-3′) | |
|---|---|---|
| Forward | Reverse | |
| Actb | AGCCTGGATGGCTACGTACA | CCTCTGAACCCTAAGGCCAA |
| Agrp | AGGACTCGTGCAGCCTTACAC | GCAGAGGTGCTAGATCCACAGAA |
| Cartpt | CCGCCTTGGCAGCTCCTT | CCGAGCCCTGGACATCTACT |
| Crh | CCGATAATCTCCATCAGTTTCCTG | TGGATCTCACCTTCCACCTTCTG |
| Gapdh | CCGTTCAGCTCTGGGATGAC | GGGCAGCCCAGAACATCAT |
| Hcrp | AGGGAGAGGCAATCCGGAGAG | GCGGCCTCAGACTCCT |
| Npy | CCCTCAGCCAGAATGCCCAA | CCGCCCGCCATGATGCTAGGTA |
| Lepr | CCAGAAGAAGAGGACCAAATATCAC | ACTTAATTTCCAAAAGCCTGAAACA |
| Ppia | TATCTGCACTGCCAAGACTGAGT | CTTCTTGCTGGTCTTGCCATTCC |
| Pmch | CTTCTACGTTCCTGATGGACTT | ATGCTGGCCTTTTCTTTGTTT |
| Pomc | GCAAGCCAGCAGGTTGCT | ATAGACGTGTGGAGCTGGTGC |
| Tnf | GGTTGTCTTTGAGATCCATGC | TCTCAAAACTCGAGTGACAAGC |