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. 2022 Sep 8;10(9):2232. doi: 10.3390/biomedicines10092232

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of systemic l-NAME treatment on reactive oxygen species (ROS) levels and expression of proteins involved in ROS production and degradation. Data showing ROS levels in RVLM (A) and MDA levels in plasma (B) of young (8 weeks, open circles or squares) or adult (20 weeks, filled circles or squares) rats after i.p. infusion of saline or l-NAME (10 mg/kg/day) for 14 days. Also shown are representative gels (insets) and densitometric analysis of results from Western blot changes in protein expression of pg91^phox, p22^phox, p47^phox, and p67^phox (C) or SOD2 and Nrf2 (D), as well as enzyme activity of NADPH oxidase and total antioxidants (E) in RVLM 14 days after saline or l-NAME treatment. Data on protein expression are normalized to the respective saline control value, which is set to 1.0. Data are presented as mean ± SD, n = 6 per group. * p < 0.05 versus corresponding saline-treated group in unpaired Student’s t-test.