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. 2022 Sep 8;10(9):2232. doi: 10.3390/biomedicines10092232

Figure 5.

Figure 5

Effect of systemic l-NAME treatment on mitochondrial electron transport chain enzyme activity and NO synthase (NOS) uncoupling in RVLM: (A) Enzyme activities of NADH cytochrome c reductase (NCCR, marker for coupling capacity between complexes I and III), succinate cytochrome c reductase (SCCR, marker for coupling capacity between complexes II and III), and cytochrome c oxidase (CCO, marker for complexes IV) in RVLM of young (8 weeks, open circles or squares) or adult (20 weeks, filled circles or squares) rats after i.p. infusion of saline or l-NAME (10 mg/kg/day). (B) Representative gels (insets) and densitometric analysis of results from Western blot showing changes in the ratio of dimers versus monomers of eNOS and nNOS protein in RVLM of young and adult rats after saline or l-NAME treatment. Data on protein expression were normalized to the respective saline control value, which is set to 100%. Data are presented as mean ± SD, n = 6 per group. * p < 0.05 versus corresponding saline-treated group, and # p < 0.05 versus saline-treated young rats in unpaired Student’s t-test.