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. 2022 Sep 8;11(18):2807. doi: 10.3390/cells11182807

Figure 3.

Figure 3

Effect of sphingomyelin on TPC2 and TRPML1 activities. (A,B) Representative measurements (I/Cm -V traces) of transiently transfected PM variants of hTPC2 (A) and hTRPML1 (B) in HEK cells. Channels were activated by small molecule agonists (10 µM TPC2-A1P and 10 µM ML-SA1), respectively. Subsequently, bath solution was completely exchanged for a solution containing agonist plus 20 µM sphingomyelin (SM). Bath solution contained: 138 mM NaCl, 6 mM KCl, 2 mM MgCl2, 2 mM CaCl2, 10 mM HEPES, and 5.5 mM D-glucose (adjusted to pH 7.4 with NaOH) and pipette solution contained 140 mM K-MSA, 5 mM KOH, 4 mM NaCl, 0.39 mM CaCl2, 1 mM EGTA and 20 mM HEPES (pH was adjusted with KOH to 7.2).