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. 2022 Sep 7;11(18):2799. doi: 10.3390/cells11182799

Figure 1.

Figure 1

Human lipoaspirate originate performing AD-MSCs in vitro: (a) photomicrograph of fibroblastoid elements adhering to plastic after adipose tissue digestion (Ph1, scale bar = 100 μm); (b) AD-MSC cumulative population doubling after passage 3 (P3); (c) representative immunophenotypical characterizations of AD-MSCs, with cells positive for CD90, CD105, and CD73 and negative for CD14, CD45, HLA-DR, CD31, and CD146 (isotype control in gray; on the right, the table shows the mean values ± SEM of antigen expressed on AD-MSCs by FACS; (d) differentiation assays on expanded cells after passage P3; osteogenic differentiation visualized by Alizarin Red staining after 14 days of induction and a corresponding noninduced control (upper panels), adipogenic differentiation after 10 days of induction visualized by Oil Red O staining and a corresponding noninduced control (middle panel), and three-dimensional chondrogenic differentiation after 21 days of induction in pellet culture stained with Alcian blue and a corresponding noninduced control (lower panel), scale bar = 100 μm; (e) AD-MSCs expressed GFP after retroviral transduction visualized by both GFP filter fluorescence (upper panel) and ph1 (lower panel), scale bar = 100 μm.