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. 2022 Sep 14;12(9):756. doi: 10.3390/bios12090756

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Discriminating power of IL-6 VFA and commercial ELISA. (A–C) IL-6 levels in 20 RA serum and 20 HC serum as assayed using a commercial IL-6 ELISA, IL-6 VFA with IS readouts, and IL-6 VFA with OS readouts. IL-6 level group comparisons were analyzed using the Mann–Whitney U-test (** p < 0.01). IL-6 levels in RA were significantly higher than in HC by all 3 readouts. (D) The correlation between VFA by IS and VFA by OS showed a Spearman correlation coefficient of 0.88, p < 0.0001. (E) The correlation between IL-6 by ELISA and VFA by OS is plotted with a Spearman correlation coefficient = 0.56. p = 0.0002. RA: rheumatoid arthritis; HC: healthy control IS: imaging score; OS: observer score.