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. 2022 Sep 14;14(18):4463. doi: 10.3390/cancers14184463

Figure 4.

Figure 4

Effect of anti-PTK7 mAbs on the chemotactic migration of HUVECs. Chemotactic migration of cells treated with mAb-32, mAb-43, and mAb-52 (3 and 10 μg/mL) or sPTK7 (4 μg/mL) was analyzed for 4 h. Cells that migrated to the bottom surface of the well were counted after crystal violet staining. Magnification = ×40. “–”: no treatment with anti-PTK7 mAb or sPTK7. The graph shows the number of migrated cells in the mAb-treated and sPTK7-treated groups relative to that in the VEGF-alone group (n = 6). Data are represented as mean ± standard deviation. *** p < 0.001 vs. VEGF-alone control. The absence of the p value between antibody-treated groups indicates statistical insignificance.