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. 2022 Sep 14;14(18):4463. doi: 10.3390/cancers14184463

Figure 8.

Figure 8

Effect of PTK7 mAbs on VEGF-induced angiogenesis in vivo. Matrigel plug assay was performed to analyze angiogenesis in vivo. Mice were subcutaneously injected with growth-factor-reduced Matrigel (0.5 mL) with or without mouse VEGF (500 ng/mL) in the presence of mAb-32 or mAb-43 (3 or 10 μg/mL). After 12 days, the mice were sacrificed and the plugs were recovered. (A) Images of the plugs are shown (n = 5). “–”: no treatment with anti-PTK7 mAb. (B) Hemoglobin content in the plugs was measured using Drabkin’s Reagent Kit 525 to quantify the degree of angiogenesis. Data are represented as mean ± standard deviation. *** p < 0.001 vs. VEGF-alone control. The absence of the p value between antibody-treated groups indicates statistical insignificance.