Figure 10.

Effect of PTK7 mAbs on PTK7-KDR interaction. HEK293 cells overexpressing PTK7-FLAG and KDR-HA were incubated with mAb-32 or mAb-43 (10 μg/mL) for 2 h. Bovine IgG (10 μg/mL) and sPTK7 (4 μg/mL) were used as negative and positive controls, respectively. Cell lysates and proteins pulled down with anti-FLAG antibodies were subjected to SDS-PAGE and Western blotting with anti-HA antibodies to detect KDR, anti-FLAG antibodies to detect PTK7, and anti-GAPDH antibodies. “+” and “–” for PTK7-FLAG or KDR-HA indicate expression and no expression of PTK7-FLAG or KDR-HA, respectively. “–” just above the western blot indicates no treatment with anti-PTK7 mAb or sPTK7.