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. 2022 Sep 10;11(18):2832. doi: 10.3390/cells11182832

Figure 1.

Figure 1

Vgll4 transcript variants and their protein products: (A) schematic view of mouse Vgll4 transcript variants. Black bars indicate non-translated mRNA sequences. Red bars indicate protein-coding sequences. The protein ID encoded by each variant was listed; (B) N-termini and C-termini alignments of mouse VGLL4 protein isoforms. N-termini alignment: blue and red letters indicate exon 2- and exon3-encoded amino acid residues, respectively. C-termini alignment: green and purple letters indicate TDU1 and TUD2 domains, respectively; (C) qRT-PCR measurement of three Vgll4 transcript variants. Each dot indicates a data point from one individual animal. Total RNA from P1 and P28 hearts were used for qRT-PCR test. mRNA level of each isoform was normalized to Gapdh. One-way ANOVA test, ** p < 0.01, *** p < 0.001. N = 3.