FIG. 2.
Sequence of the trfAp-trbAp-trbBp region. Coordinates refer to the complete sequence of RK2, available as GenBank accession no. LZ7758. −35 and −10 regions of relevant promoters are indicated. OA, KorA operator; OB, KorB operator; SD, Shine-Dalgarno sequence. Asterisks and alternative letters show mutations in either the putative degenerate KorB operator (first three, from left to right) or in the −10 region of the trbBp region (fourth and fifth). The primers used for amplification of promoter fragments and TrbA deletions by PCR (described in Materials and Methods) correspond to the following coordinates: 1, 18190 to 18212; 2, 18259 to 18278; 3, 18242 to 18263; 4, 18449 to 18431; 5, 18498 to 18477; 6, 18506 to 18489; 7, 18514 to 18493; 8, 18518 to 18501; 9, 18527 to 18508; 10, 18554 to 18534; 11, 18716 to 18735; 12, 18742 to 18759; 13, 18790 to 18776; 14, 18915 to 18896; and 15, 18397 to 18414. orf, open reading frame.