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Western blot analysis of C-terminally deleted TrbA derivatives, carried out as described in Materials and Methods. Lanes: 1, pMZT24 (TrbA wt); 2, pMZT46 (C-Δ15); 3, pMZT47 (C-Δ18); 4, pMZT48 (C-Δ33); 5, pMZT43 (C-Δ8); 6, pMZT44 (C-Δ11); 7, pMZT45 (C-Δ13); 8, pGBT30 (no insert).
