TABLE 3.

Mapping of sites needed for repression of trbBp by KorB and TrbA^a

Reporter plasmid	KorBb					TrbAd
	Presence (+) or absence (−) of repressor	Overnight culture (+ 0.05 mM IPTG)		Logarithmic culture (+ 0.5 mM IPTG)		Presence (+) or absence (−) of repressor	Overnight culture (+ 0.05 mM IPTG)		Logarithmic culture (+ 0.5 mM IPTG)
		XylE unitsa	Rc	XylE unitse	Rc		XylE unitse	Rc	XylE unitse	Rc
pGBT63	+	0.53	24.3	0.053	48.9	+	0.84	18	0.39	3.54
	−	12.9		2.59		−	15.1		1.38
pGBT66	+	0.21	31.4	0.032	59.6	+	0.81	14.7	0.34	4.3
	−	6.6		1.91		−	11.9		1.46
pMZT37	+	0.23	22.6	0.025	78.8	+	0.93	12.7	0.25	3
	−	5.2		1.97		−	11.8		0.74
pMZT38	+	0.22	31.3	0.02	112	+	0.8	14.4	0.23	5.04
	−	6.9		2.24		−	11.5		1.16
pMZT39	+	0.29	19.3	0.025	96	+	0.99	13.9	0.43	3.6
	−	5.6		2.4		−	13.8		1.56
pMZT14	+	0.009	38.9	0.0045	37.8	+	0.45	1.0	0.06	1.5
	−	0.35		0.17		−	0.45		0.09
pMZT40	+	4.22	1.2	0.94	2.6	+	0.77	15.1	0.27	3.9
	−	5.0		2.45		−	11.6		1.05
pMZT41	+	4.31	1.2	1.1	1.8	+	0.45	17	0.24	5.6
	−	5.1		2.01		−	7.64		1.34
pMZT42	+	3.27	1.4	0.82	2.1	+	0.93	17.5	0.12	7.3
	−	4.6		1.74		−	16.3		0.84

^aResults come from at least three experiments. The RK2 DNA present in the reporter plasmids is shown in Fig. 3. 

^bKorB was expressed by plasmid pDM1.21, while the expression vector pDM1.2 was used to replace it for the no-repressor control. 

^cRepression ratio, calculated as XylE units with repressor/XylE units without repressor. 

^dTrbA was expressed by plasmid pMZT24, while the expression vector pGBT30 was used to replace it for the no-repressor control. 

^eFor clarity, the standard deviation values, which were generally in the same range as shown in the other tables, were omitted.