Figure 6.

Bond tension acting on β₂-integrins modulates superoxide production in neutrophils. (a) Schematic of neutrophils bound to 12pN or 54pN TGT presenting ICAM-1 in presence and absence of HA β₂-integrin activated with fMLP. Shear force produced by oscillatory mixing greater than the TGT tolerance results in rupture. (b) Max ROS production for neutrophils bound to 54pN or 12pN TGT + ICAM-1 in the presence or absence of fMLP or CD18 blocking antibody IB4. All data are represented as mean +/− SEM, n = 3, bars indicate significance between mean values. Note that 12pN TGT + ICAM-1 did not register a significant increase compared to fMLP stimulation in absence of IB4.