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. 2022 Sep 9;14(18):4398. doi: 10.3390/cancers14184398

Figure 1.

Figure 1

The effect of Farnesoid X receptor (FXR) overexpression on cholesterol content and cholesterol biosynthesis-related protein expression. (A) FXR was overexpressed (FXR-O) in TSGH8301 and T24 cells for 72 h, and the cholesterol content was measured by a cholesterol cell-based assay. (B) The level of cholesterol secreted into the conditioned medium of TSGH8301 and T24 cells was analyzed by ELISA. (C) FXR was overexpressed in TSGH8301 and T24 cells for 72 h, and p-AMPK, AMPK, SREBP2 and HMGCR protein expression was examined by Western blotting. GAPDH was a loading control. The relative quantitative analysis of these proteins was shown by bar graphs. * p < 0.05; ** p < 0.01 compared with the control group. Scale bar = 50 μm. The original blots are shown in Figure S6A.