Figure 3.

The effect of dorsomorphin on invasion and angiogenesis of human bladder cancer T24 cells after FXR overexpression. (A) The invasive ability of muscle-invasive FXR-O T24 cells treated with or without dorsomorphin was analyzed by Transwell assay with Matrigel for 18 h. The cells were stained and imaged. The lower panel shows the quantitative results. (B) HUVECs were cultured with control or FXR-O conditioned medium with or without dorsomorphin for 6 h. The number of branch points and lengths of endothelial cell networks were analyzed. The quantitative results of relative branch point numbers and tube lengths were shown as below. ** p < 0.01 compared to the control group; # p < 0.05 compared to the FXR-O group. Scale bar = 200 μm.