Figure 1.

Effect of JQ1 on the hypoxia status of NB cells in vitro. (A) Western blot test showing the protein expression of HIF-1α and CA9 in NHO2A, CHP-134, and SIMA cells cultured under normoxia (N) or hypoxia and treated with JQ1 at the indicated concentration. Actin was used as a loading control. The quantification of band intensity corresponding to HIF-1α and CA9 in treated hypoxic cells is reported compared to untreated hypoxic cells and normoxic cells, respectively. (B) RT-qPCR measurement of CA9 mRNA in cells described in (A). Bars represent means from three independent experiments ± SEM. Statistically significant difference was calculated by unpaired two-tailed Student’s t-test (* p < 0.05, ** p <0.01; and *** p < 0.001).