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. 2022 Sep 15;14(18):4478. doi: 10.3390/cancers14184478

Figure 2.

Figure 2

Isolation and localization of L1 and TFF1 in CRC cell clones overexpressing both L1 and TFF1. (A) Serial dilutions of cell extracts analyzed by immuno-dot-blotting using LS 174T CRC cell clones overexpressing both L1 (L1) and TFF1 (L1 + TFF1 cl1 and cl2); (B) densitometric analysis of the dot blots shown in (A); (C) localization of L1 and TFF1 in CRC cell clones overexpressing both L1 (red) and TFF1 (green) by double immunofluorescence microscopy. Nuclei were stained with DAPI (blue). * p < 0.05, ** p < 0.01. The uncropped blots are shown in Supplementary File S1.