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. 2022 Aug 26;12(9):1138. doi: 10.3390/brainsci12091138

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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RPH3A alters the proliferation of LGG cells. (A). Relative expression of RPH3A in LGG and normal brain tissues. (B,C) Transfection efficiency of the RPH3A in SW1783 and SW1088 cells, measured by qRT-PCR and western blotting. (D) RPH3A-containing colonies showed reduced viability when compared to empty vector-containing colonies. (E) In CCK-8 assays, LGG cells expressing up-regulated RPH3A showed decreased cell viability. Means and standard deviations are represented as means and standard deviations. * p < 0.05, and ** p < 0.01 compare vs. Empty vector group, *** p < 0.001 compare vs. normal group. ns p > 0.05 compare vs control group.