Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Sep 19;11(18):2927. doi: 10.3390/cells11182927

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

IAL inhibits the release of pro-inflammatory mediators in BV2 cells. (A) The effect of IAL on BV2 cell viability was examined with a CCK-8 assay. (B–E) The mRNA expression of the pro-inflammatory mediators IL-6, TNF-α, iNOS and COX2 was detected with a real-time PCR assay. (F,G) The protein expression of the pro-inflammatory mediators IL-6 and TNF-α was detected with an ELISA assay. (H–J) The protein expression of the pro-inflammatory mediators (iNOS and COX2) was detected with a Western blot assay. Results are shown as means ± SEM (n = 3). ns stands for no significant difference. ^## p < 0.01, * p < 0.05 and ** p < 0.01 stand for biologically significant differences.