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. 2022 Sep 19;11(18):2927. doi: 10.3390/cells11182927

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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IAL treatment protects SN4741 from microglial activation-mediated neurotoxicity. (A) Conditioned medium preparation flow chart. (B) The effect of conditioned medium on SN4741 cell viability was examined using the CCK-8 method. (C) The effect of conditioned medium on cellular LDH release was examined using the LDH method. (D) The effect of conditioned medium on SN4741 cell death was examined using Tunel staining. Results are shown as means ± SEM (n = 3). * p < 0.05 and ** p < 0.01 stand for biologically significant differences.