Figure 2.

SYN or ALLO BMSC intramuscular injection. (A) Experimental procedure. (B) Whole muscle analysis and (C) optical sections of regenerating mouse skeletal muscles showing SYN and ALLO BMSC localization 14 days after muscle injury; images obtained with the iDISCO+ method; asterisks indicate localization of cells; SYN BMSCs or ALLO BMSCs are shown in green. Dashed lines visible on images in (B) indicate the plane of collection of optical sections shown in (C). For visualization of the entire muscle, myoglobin autofluorescence was collected (for each muscle treatment n = 2).