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. 2022 Sep 12;11(18):2843. doi: 10.3390/cells11182843

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Luminex assay analysis of regenerating muscles injected with NaCl or SYN CM or SYN or ALLO BMSCs. (A) Concentration of pro-inflammatory factors: CXCL1, CCL2, TNF-α, IL-4, IL-6, IL-13, IL-33, and IFN-γ in muscles injected with NaCl (control, white bars) or SYN CM (black bars) or SYN BMSCs (light grey bars) or ALLO BMSCs (dark grey bars) on days 7, 14, and 30 after injury. (B) Concentration of anti-inflammatory factors: IL-10 and IGF-I in muscles injected with NaCl (control, white bars) or SYN CM (black bars) or SYN BMSCs (light grey bars) or ALLO BMSCs (dark grey bars) on days 7, 14, and 30 after injury. Data are presented as means of three independent experiments with standard deviations. (C) Graphical summary of the Luminex assay analysis. The darker hue represents a higher relative concentration for each factor compared to the sample in which the highest concentration was found. Data found to be statistically significant are underlined on graphs and are marked with asterisks (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Luminex assay analysis of regenerating muscles injected with NaCl or SYN CM or SYN or ALLO BMSCs. (A) Concentration of pro-inflammatory factors: CXCL1, CCL2, TNF-α, IL-4, IL-6, IL-13, IL-33, and IFN-γ in muscles injected with NaCl (control, white bars) or SYN CM (black bars) or SYN BMSCs (light grey bars) or ALLO BMSCs (dark grey bars) on days 7, 14, and 30 after injury. (B) Concentration of anti-inflammatory factors: IL-10 and IGF-I in muscles injected with NaCl (control, white bars) or SYN CM (black bars) or SYN BMSCs (light grey bars) or ALLO BMSCs (dark grey bars) on days 7, 14, and 30 after injury. Data are presented as means of three independent experiments with standard deviations. (C) Graphical summary of the Luminex assay analysis. The darker hue represents a higher relative concentration for each factor compared to the sample in which the highest concentration was found. Data found to be statistically significant are underlined on graphs and are marked with asterisks (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).