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. 2022 Aug 28;9(9):1305. doi: 10.3390/children9091305

Figure 3.

Figure 3

Comparisons of control vs. intermittent hypoxia (IH)-treated islets in the quantity of insulin secretion and the chloride levels measured from isolated primary islets at different glucose levels. IH treatment was performed 12 h prior to this set of assays. Insulin secretion quantified by Enzyme-linked immunosorbent assay (ELISA) (A), KCC2 levels compared using Western blots (B) and chloride content quantified by a colorimetric assay (C) following IH exposure in 2 mM and 16 mM glucose media. No influence from the different glucose levels in media was noted. Western blots for Glucose transporter 2 (Glut2) and KCC2 show a marked difference between the control and IH in both glucose media. Levels of sulfonylurea receptor 1 (Sur1) and ATP-sensitive K+ channel (Kir6.2) showed no marked changes. Addition of tolbutamide to 2 mM increased insulin secretion (D). Western blots showed minimal changes in response to tolbutamide (E) and the chloride levels showed no changes (F). Full-length blots are found in the Supplementary Information, Figure S1. * indicates significantly.