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. 2022 Sep 19;14(18):4524. doi: 10.3390/cancers14184524

Figure 2.

Figure 2

Transient overexpression of circular RNA hsa_circ_0062682 promoted the oncogenic potential of HCC model cell lines. (A) Relative expression of hsa_circ_0062682 in transiently overexpressing Huh-7 (up) (n = 3) and HepG2 (down) cell line (n = 3); (B) colony forming assay in hsa_circ_0062682-overexpressing Huh-7 (left) (n = 3) (10,000 cells) and HepG2 (right) cell lines (n = 3) (5000 cells); (C) proliferation of hsa_circ_0062682-overexpressing Huh-7 (up) (n = 4) and HepG2 (down) cell lines (n = 4); (D) wound healing assay of hsa_circ_0062682-overexpressing Huh-7 (up) (n = 4) and HepG2 (down) (n = 4) cell lines; representative images of Huh-7 migration are shown on the right; representative images of HepG2 migration can be found in Supplementary Materials; pRR–empty plasmid, pRR-62682–plasmid for hsa_circ_0062682 overexpression; results are represented as averages and error bars represent standard deviation. * p-value ≤ 0.05; ** p-value ≤ 0.01; *** p-value ≤ 0.001; **** p-value ≤ 0.0001, Student’s t-test.