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. 2022 Sep 19;14(18):4524. doi: 10.3390/cancers14184524

Figure 7.

Figure 7

Hsa_circ_0062682 binds to YBX1 and its effect is cell-context dependent. (A) Confirmation of a specific pulldown from RNA immunoprecipitation of YBX1 by Western blotting (up); enrichment of RNA species from RNA immunoprecipitation by RT-qPCR (n = 3) (down); (B) comparison of RNA immunoprecipitation enrichment between Huh-7 OE and Huh-7 control cell line normalized to expression levels in IgG fractions (top) or expression levels in the input (bottom) (n = 3); (C) enrichment of RNA species in biotinylated oligonucleotide pulldowns normalized to biotinylated anti-GFP oligonucleotide pulldown; antisense (up), sense (down) (n = 3); (D) viability of sorafenib treated KD cell lines measured by colourimetric CCK-8 assay (n = 4); (E) hnRNP K localization in hsa_circ_0062682 stable cell lines identified by Western blotting (n = 3); OE: overexpression; KD: knockdown; cyto: cytoplasmic fraction; nuc: nuclear fraction. Results are represented as averages and error bars represent standard deviation. * p-value ≤ 0.05; ** p-value ≤ 0.01; *** p-value ≤ 0.001; **** p-value ≤ 0.0001; n.s.: not significant, Student’s t-test. The uncropped blots are shown in Supplementary Data.