TABLE 1.
Growth responses of pha mutants on plates
| Mutant | Growth responsea
on:
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Succinate | Pyruvate | Methanol plus succinate | Methanol | Ethanol | Ethylamine | Methanol plus glyoxylate or glycolate | Ethylamine plus glyoxylate or glycolate | Formate | β-Hydroxybutyrate | |
| phaA | ++ | ++ | ++ | − | − | − | − | − | − | − |
| phaB | ++ | ++ | ++ | + | − | − | ++ | ++ | + | − |
| phaCb | ++ | ++ | ++ | + | + | + | NT | NT | + | + |
a
++, Similar to wild type; +, decrease compared to wild type; −, no growth; NT, not tested.
b
Generates second site suppressor mutants at high frequency.