Table 2.

Culture models for studying CAFs.

Culture Model	References
Generation of cancer spheroid and 3D mucosal sheet model	[271,272,273,274,275,276]
Cell viability assays
Visualization of hypoxia in the 3D cell-sheet model
Green fluorescent protein gene transfection
Reverse transcription-quantitative polymerase chain reaction and immunoblotting
CAFs sources
Primary CAFs (mouse/human)	[277,278]
LX-2	[279,280,281,282,283,284,285,286]
Primary HSCs	[287,288]
3T3-NIH	[288]
Cell lines
HCT-116	[224,279]
LS174T	[279]
HT-29	[224,289]
CT-26	[287,288]