TABLE 3.

Nucleoid scoring and sporulation frequency of B. subtilis mutants implicated in chromosome dimer resolution

Strain (relevant genotype)	Nucleoid scoring		Sporulation frequencyb
	Cells with partition defects (%)a	Anucleate (%)	Proportion phase brightc	Relative sporulationd
BR151 (parent)	0.56	0	0.77	1.0
SL7355 (codV::neo)	14	0.83	0.58	0.75
SL7376 (ripX::cat)	25	1.6	0.52	0.67
SL8420 (Δdif::neo)	25	1.3	0.52	0.67
SL7513 (ΔSPβ parent)	0.66	0	0.62	1.0
SL7752 (ΔSPβ codV::neo)	29	9.2	0.38	0.61
SL7519 (ΔSPβ ripX::cat)	41	11	0.38	0.61
SL8449 (ΔSPβ Δdif::neo)	36	7.9	0.19	0.31
SL8081 (yopP::spc)	1.6	0	0.68	0.88
SL8101 (yopP::spc codV::neo)	31	2.9	0.54	0.70
SL8172 (yopP::spc ripX::cat)	41	4.7	0.51	0.66
SL8540 (yopP::spc Δdif::neo)	40	3.3	0.44	0.57
SL7375 (spoIIIE::spc)	1.3	0.1	NDe	ND
SL8562 (ytpT::spc)	0.6	0.1	ND	ND
SL8626 (spoIIIE::spc ytpT::erm)	6.2	0	ND	ND

^aPercentage shown is the sum of all four abnormal nucleoid phenotypes (includes anucleate cells) shown and described in Fig. 1. At least 600 cells were scored per strain. 

^bScoring was done at t₂₀ in a Petroff-Hauser counting chamber by phase-contrast microscopy. 

^cResults presented are the average of two independent experiments, > 1,000 phase-bright spores and phase-dark cells were scored per strain. A 95% confidence limit for all proportions was found between (±) 0.02 and 0.03. 

^dRelative sporulation is presented with respect to the appropriate parent strain (i.e., SL7513 for ΔSPβ, BR151 for other strains). 

^eND, not determined.