TABLE 4.
Integration efficiency of Bsdif-containing plasmids in various FtsK-like mutant backgrounds
| Strain (relevant genotype) | No. of antibiotic-resistant transformants using the DNA indicateda
|
|||||
|---|---|---|---|---|---|---|
| No DNA | pSAS141 | pSAS142 | pSAS143 | pSAS144 | pECE85 | |
| SL7360 (recA::neo) | 0 | 551 | 449 | 165 | 107 | 1,532 |
| SL7412 (recA::neo spoIIIE::spc) | 0 | 398 | 247 | 114 | 50 | 1,862 |
| SL8576 (recA::neo ytpT::spc) | 0 | 626 | 522 | 240 | 96 | 2,280 |
| SL8629 (recA::neo spoIIIE::spc ytpT::erm) | 0 | 378 | 291 | 144 | 16 | 1,240 |
a
Approximately 8 μg per ml of competent culture was used. A total of 0.3 ml of culture was plated in each case. The pSAS series of plasmids contain various portions of the Bsdif region cloned into nonautonomously replicating plasmids. The Positive control DNA used in these experiments was the shuttle plasmid pECE85.