Figure 1.

Retentions and dynamics of TCR expression in Jurkat and NK92 cells. (A) Confirmation of TCR expression in Jurkat cells. Three days after transduction, cells were harvested and stained for flow cytometry to assess the expression of TCR on the surface of Jurkat cells. Live cells were pre-gated during the analysis of acquired data. Three independent experiments were performed. The ** indicates a statistically significant p < 0.01. (B) Dynamics of transgene expression in NK92 cells. NK92 cells were transduced with TCR and then the day after they were transduced with CD3 subunits lentiviral vector. Three- and seven-days post-transduction, cells were harvested and stained for flow cytometry to see the expression of TCR. Live cells were pre-gated during the analysis of acquired data. Four experiments with sequential transduction and single transduction were carried out. ns indicates not significant. * p < 0.05.