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. 2022 Aug 24;44(9):3809–3821. doi: 10.3390/cimb44090261

Figure 1.

Figure 1

TGF-β receptor signaling agonist T74 blocks DC maturation. (a) Whole cell lysates pre-pared from T74-untreated DCs (NT) and T74-treated DCs (T74) were evaluated by western blotting for pTGFBRI (TβR1), TGFBRI. Values were normalized to GAPDH expression, n = 3. Data are representative of three independent experiments. (b) Pro-inflammatory cytokine secretion by T74-treated DCs (T74-DCs) was analyzed by ELISA, n = 5. (c) DCs were stained with fluorescence-conjugated antibodies specific for the indicated molecules and analyzed by flow cytometry. The histograms are representative of five independent mice. The bar graph shows the percentage of cells positive for each marker, n = 5. Data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. N/D: not detected.