Table 4.

DNA extraction conditions for 120 samples used for validation of the gene-editing test.

Sample	Condition	Number of Samples Used
Hair roots	Extracted/Purified	46
Hair roots	WGA, Extracted/Purified *	5 ***
Blood	Extracted/Purified	50 ****
Edited cell_1	Extracted/Purified	1
Edited cell_1	WGA, Extracted/Purified *	2
Edited cell_1	WGA **	1
Edited cell_2	WGA, Extracted/Purified *	4
Edited cell_2	WGA **	2
Edited cell_3	WGA, Extracted/Purified *	2
Edited cell_3	WGA **	1
Edited cell_4	WGA, Extracted/Purified *	4
Edited cell_4	WGA **	2

*: Amplified genomic DNA amplified was purified using the DNeasy Blood and Tissue Kit. **: Amplified genomic DNA was directly used for library preparation. ***: The five samples were duplicates of samples included in the group of 46. ****: Four of the fifty blood samples were taken from horses that were also typed using 46 hair root samples.