. 2022 Aug 25;13(9):1528. doi: 10.3390/genes13091528

Table 3.

Validation of mintRULS predictions in case of mutations in the seed region of miRNAs or in the target gene itself. Upper quartile (>75th percentile), Moderate Target: Middle quartile (in between 25th and 75th percentile), and Weak Target: Lower quartile (<25th percentile).

miRNA	miRNA/Seed Mutation	Target Gene/Mutation	Result in Reference	mintRULS Prediction		Reference
miRNA	miRNA/Seed Mutation	Target Gene/Mutation	Result in Reference	Quartile	Class	Reference
hsa-miR-124-3p	UAAGGCACGCGGUGAAUGCCAA	Parp-1 (WT)	Target	Upper	Strong Target	[73]
		Mut1: PARP-1 (CC > GG)	No target	Lower	Weak Target
		Mut2: PARP-1 (TG > CA)	No target	Lower	Weak Target
		Mut3: PARP-1 (GC > AA)	No target	Lower	Weak Target
		Mut4: deletion (ΔGC)	No target	Middle	Moderate Target
cel-let-7-3p	AU[G/A]CAA	LIN-41	WT: Target	Upper	Strong Target	[74]
cel-let-7-3p	AU[G/A]CAA	LIN-41	Mutation: No Target	Lower *	Weak Target *	[74]
hsa-miR-662	CCCAC[G/A]U	KLLN	Disrupted (∆S = −0.51)	Upper	Strong Target	PolymiRTS database
		KLLN	Disrupted (∆S = −0.51)	Lower *	Weak Target *	PolymiRTS database
		PATE4	Disrupted (∆S = −0.45)	Upper	Strong Target	PolymiRTS database
		PATE4	Disrupted (∆S = −0.45)	Lower *	Weak Target *	PolymiRTS database
hsa-miR-125a-5p	CCCUGA[G/U]	ZMYM3	Disrupted (∆S = −0.31)	Upper	Strong Target	PolymiRTS database
		ZMYM3	Disrupted (∆S = −0.31)	Lower *	Moderate Target *	PolymiRTS database
		PRRC1	Disrupted (∆S = −0.45)	Upper	Strong Target	PolymiRTS database
		PRRC1	Disrupted (∆S = −0.45)	Lower *	Weak Target *	PolymiRTS database
		AQPEP	Disrupted (∆S = −0.42)	Upper	Strong Target	PolymiRTS database
		AQPEP	Disrupted (∆S = −0.42)	Lower *	Weak Target *	PolymiRTS database
hsa-miR-645	[C/G]UAGGCU	COL4A4	Disrupted (∆S = −0.38)	Upper	Strong Target	PolymiRTS database
		COL4A4	Disrupted (∆S = −0.38)	Middle *	Moderate Target *	PolymiRTS database
		MAOA	Disrupted (∆S = −0.4)	Upper	Strong Target	PolymiRTS database
		MAOA	Disrupted (∆S = −0.4)	Lower *	Weak Target *	PolymiRTS database
		IL4R	Disrupted (∆S = −0.42)	Upper	Strong Target	PolymiRTS database
		IL4R	Disrupted (∆S = −0.42)	Lower *	Weak Target *	PolymiRTS database
hsa-miR-146a-3p		CP	Disrupted (∆S = −0.57)	Upper	Strong Target	PolymiRTS database
		CP	Disrupted (∆S = −0.57)	Lower *	Weak Target *	PolymiRTS database
		ABCB1	Disrupted (∆S = −0.35)	Upper	Strong Target	PolymiRTS database
		ABCB1	Disrupted (∆S = −0.35)	Lower *	Weak Target *	PolymiRTS database
mmu-miR-342-5p	[G/-]GGGUGC	PIGU	Disrupted (∆S = −0.46)	Upper	Strong Target	PolymiRTS database
		PIGU	Disrupted (∆S = −0.46)	Lower *	Weak Target *	PolymiRTS database
		RASL10B	Disrupted (∆S = −0.5)	Middle	Moderate Target	PolymiRTS database
		RASL10B	Disrupted (∆S = −0.5)	Lower *	Weak Target *	PolymiRTS database
		MCU	Disrupted (∆S = −0.54)	Upper	Strong Target	PolymiRTS database
		MCU	Disrupted (∆S = −0.54)	Lower *	Weak Target *	PolymiRTS database
mmu-miR-690	AAGGCU[A/G]	CNOT6	Disrupted (∆S = −0.3)	Upper	Strong Target	PolymiRTS database
		CNOT6	Disrupted (∆S = −0.3)	Lower *	Weak Target *	PolymiRTS database
		ELOVL4	Disrupted (∆S = −0.35)	Upper	Strong Target	PolymiRTS database
		ELOVL4	Disrupted (∆S = −0.35)	Lower *	Weak Target *	PolymiRTS database
		RBBP5	Disrupted (∆S = −0.34)	Upper	Strong Target	PolymiRTS database
		RBBP5	Disrupted (∆S = −0.34)	Middle *	Moderate Target *	PolymiRTS database
mmu-miR-743a-3p	AAAGAC[A/G]	MXI1	Disrupted (∆S = −0.33)	Upper	Strong Target	PolymiRTS database
		MXI1	Disrupted (∆S = −0.33)	Lower *	Weak Target *	PolymiRTS database
		PRRG3	Disrupted (∆S = −0.51)	Upper	Strong Target	PolymiRTS database
		PRRG3	Disrupted (∆S = −0.51)	Lower *	Weak Target *	PolymiRTS database
		MBNL3	Disrupted (∆S = −0.43)	Upper	Strong Target	PolymiRTS database
		MBNL3	Disrupted (∆S = −0.43)	Lower *	Weak Target *	PolymiRTS database

Higher value of the context+ score difference (∆S) indicates an increased likelihood disruption of interactions between miRNA and target gene. * Entries for mutation in miRNAs. The values without * represents WT cases.