Table 2.
Characteristics of differentially interacting proteins with plasma membrane proteins of IPEC-J2 after PEDV infection, as identified by the PEDV S1 polyclonal antibody.
| Group A | No. | Protein ID | Names | Major Protein Function | Abbreviation | Coverage (%) | Length | Mass | #Unique Peptide |
| 1 | tr|F1SGG3_PIG | Keratin 1 | Intermediate filament | KRT1 | 9.221 | 624 | 64,874 | 6 | |
| 2 | tr|A5A759_PIG | Keratin 2A | Intermediate filament | KRT2A | 5.321 | 639 | 65,663 | 3 | |
| 3 | tr|F1SGG6_PIG | Keratin 5 | Intermediate filament | KRT5 | 3.299 | 576 | 61,358.9 | 2 | |
| 5 | tr|A0A287BSX6_PIG | Keratin 14 | Intermediate filament | KRT14 | 5.765 | 503 | 54,723.5 | 2 | |
| 4 | tr|A0A287B4G4_PIG | Rac family small GTPase 1 | Regulation of actin cytoskeleton organization | RAC1 | 9.38 | 192 | 21,449.9 | 2 | |
| 6 | tr|A0A287A2M2_PIG | Keratin 80 | Intermediate filament | KRT80 | 2.309 | 433 | 48,451.4 | 1 | |
| 7 | tr|Q0PY11_PIG | Elongation factor 1-α | Promoting the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis | EEF1A | 15.25 | 59 | 6246.3 | 1 | |
| Group B | No. | Protein ID | Names | Major Protein Function | Abbreviation | Coverage (%) | Length | Mass | #Unique Peptide |
| 1 | sp|P02543|VIME_PIG | Vimentin | Intermediate filament | VIM | 17.6 | 466 | 53,667.2 | 7 | |
| 2 | tr|F1RT622_PIG | Integrin subunit α 3 | Virus receptor | ITGA3 | 2.12 | 1037 | 115,677.5 | 2 | |
| 3 | tr| K7GS94_PIG | Integrin β | Virus receptor | ITGB1 | 1.25 | 853 | 93,820 | 1 | |
| 4 | tr|A0A287AY77_PIG | CCR4-NOT transcription complex subunit 2 | Positive regulation of cytoplasmic mRNA processing body assembly | CNOT2 | 1.98 | 540 | 59,738 | 1 | |
| 5 | tr|B2CZF5_PIG | Monocarboxylic acid transporter 1 | Proton-coupled monocarboxylate transporter | SMCT1 | 2.59 | 501 | 54,246.8 | 1 | |
| 6 | tr|K7GSD2_PIG | Guanine nucleotide-binding protein G(s) subunit α | G-protein β/γ-subunit complex binding | GNAS | 1.74 | 632 | 69,389.4 | 1 | |
| 7 | tr|F2Y8C4_PIG | G protein-coupled receptor family C group 5 member C | G protein-coupled receptor activity | GPRC5C | 3.46 | 434 | 46,933.2 | 1 | |
| 8 | tr|F2Z565_PIG | Solute carrier family 25 | Catalyzes the exchange of ADP and ATP across the membrane | SLC25A5 | 6.38 | 298 | 32,955 | 1 | |
| 9 | tr|I3LB80_PIG | Solute carrier family 3 member 2 | L-alanine transmembrane transporter activity | SLC3A2 | 1.76 | 568 | 61,613.4 | 1 | |
| 10 | tr|K7GKA5_PIG | Magnesium transporter 1 | Protein modification, protein glycosylation | MAGT1 | 2.419 | 372 | 42,330.2 | 1 | |
| 11 | tr|A0A286ZLL3_PIG | Receptor of activated protein C kinase 1 | Ribosome binding | RACK1 | 3.623 | 276 | 30,505.2 | 1 |
Abbreviations: Group A represents the proteins identified in the complexes of membrane proteins without PEDV infection after separation from the S1 antibody. Group B represents the proteins identified in the complexes of membrane proteins with PEDV infection after separation from the S1 antibody. The main functions of each protein are reported in the UniProt database (https://www.uniprot.org/, accessed on 17 June 2022).